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Merck

C9134

α-Chymotrypsin−Agarose from bovine pancreas

lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.21.1 (BRENDA, IUBMB)
MDL number:
MFCD00130481
Specific activity:
2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)
Biological source:
bovine pancreas

Key Documents

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biological source

bovine pancreas

form

lyophilized powder

specific activity

2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

mol wt

25 kDa

UniProt accession no.

P00767

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... CTRB1(618826)

Related Categories

Application

α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

Physical form

Stabilized with lactose

Analysis Note

Protein determined by A1%/280

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 8.0 at 30 °C.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C9134-100UN: + C9134-5UN: + C9134-25UN: + C9134-VAR: + C9134-50UN: + C9134-BULK: + C9134-10UN:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
SLBT2346
066K7035
061H9545
024K7031

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Saleh A Mohamed et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 140(1), 73-80 (2004-12-29)
The present study analyzed the existence of carbohydrases in camel pancreas compared to some other ruminants. Disaccharidases (maltase, cellobiase, lactase, trehalase and sucrase), glucoamylase and alpha-amylase were detected in pancreas of camel, sheep, cow and buffalo. Enzyme levels in sheep
Ke Xu et al.
The Journal of biological chemistry, 283(49), 34337-34344 (2008-10-11)
The clinical severity of Osteogenesis Imperfecta (OI), also known as the brittle bone disease, relates to the extent of conformational changes in the collagen triple helix induced by Gly substitution mutations. The lingering question is why Gly substitutions at different
N E Labrou et al.
Biotechnology and bioengineering, 63(3), 322-332 (1999-04-01)
Molecular modeling was employed for the design of a biomimetic chimeric ligand for L-lactate dehydrogenase (LDH). This ligand is an anthraquinone monochlorotriazinyl dye comprising two moieties: (a) the ketocarboxyl biomimetic moiety, 2-(4-aminophenyl)-ethyloxamic acid, linked on the monochlorotriazine ring, mimicking the
Y Gao et al.
The Journal of biological chemistry, 276(13), 9838-9845 (2001-01-20)
Dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) on Ser/Thr residues is ubiquitous in higher eukaryotes and is analogous to protein phosphorylation. The enzyme for the addition of this modification, O-GlcNAc transferase, has been cloned from several
Vincent Dechavanne et al.
Protein expression and purification, 87(2), 100-110 (2012-11-14)
The proteasome is a multicatalytic protease complex present in all eukaryotic cells, which plays a critical role in regulating essential cellular processes. During the immune response to pathogens, stimulation by γ interferon induces the production of a special form of
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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

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