CS0260
Glutathione Assay Kit
sufficient for 700 assays
Synonym(s):
GSH Quantification Test
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 700 assays
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
human ... GSR(2936)
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General description
The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.
Application
Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.
Biochem/physiol Actions
Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.
Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues
Features and Benefits
- A quick and simple method
- The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
- The kit includes a deproteination reagent for biological samples
Kit Components Only
Product No.
Description
- Assay Buffer 5x 30 mL
- DTNB 8 mg
- DMSO 7.5 mL
- Glutathine Reductase 20 U
- L-Glutathione Reduced 300 μg
- NADPH 25 mg
- 5-Sulfosalicylic Acid 2.5 g
signalword
Danger
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F
flash_point_c
87 °C
target_organs
Eyes,Central nervous system
wgk
WGK 3
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 2
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
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