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CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

UNSPSC Code:
12352207
EC Number:
217-896-6
NACRES:
NA.32
Technique(s):
flow cytometry: suitable, protein staining: suitable
Usage:
 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)
Storage temp.:
−20°C
Detection method:
fluorometric
Shipped in:
dry ice
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Quality Segment

200

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable, protein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Biochem/physiol Actions

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Features and Benefits

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Kit Components Only

Product No.
Description
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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cart

キットコンポーネントの情報を参照してください

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