Skip to Content
Merck

D0564

Diphtheria Toxin

from Corynebacterium diphtheriae, ≥98% (SDS-PAGE), lyophilized powder, protein synthesis inhibitor

Synonym(s):

DTx

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD00163490

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Diphtheria Toxin from Corynebacterium diphtheriae, lyophilized powder, Product is in unnicked form

form

lyophilized powder

storage temp.

2-8°C

Quality Level

200

Related Categories

General description

Diphtheria toxin may be used as a toxin element in the construction of immunotoxin for cell-specific cytotoxicity. The translocation domain of bacterial toxins with a natural endosome escape mechanism has been used in the development of efficient nonviral vectors for applications in gene therapy.

Application

Diphtheria Toxin from Corynebacterium diphtheriae has been used:


  • for microglia depletion to study post-traumatic stress disorder in mice
  • intraperitoneally injected in transgenic mice to select the hybrids after fusion of transplanted hematopoietic stem and progenitor cells (HSPCs) during ablation
  • to study its effect on eosinophil lineage-committed progenitors in an eosinophil-deficient strain of mice (iPHIL)

Biochem/physiol Actions

Diphtheria toxin is a bacterial toxin produced from Corynebacterium diphtheriae. It contains three domains that have intracellular actions. The domains are involved in the intoxication of the cell, cell-surface binding and internalization into endosomes, translocating into the cytosol across the endosome membrane and inhibiting cellular protein synthesis. Diphtheria toxin is useful in constructing biotechnological tools and therapeutics. It inhibits protein synthesis by catalyzing adenosine diphosphate (ADP)-ribosylation of the target protein in eukaryotes.

Physical form

Lyophilized powder containing Tris and EDTA.

Preparation Note

Each vial, when reconstituted to 0.5 mL with sterile distilled water, contains ~1 mg of diphtheria toxin in 0.01 M Tris and 0.001 M Na2EDTA, pH 7.5.

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 1 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D0564-1MG-PW: + D0564-BULK: + D0564-1MG: + D0564-VAR: + D0564-1MG-LBL:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCS8526
MKCS1028
MKCQ9499
MKCR5571
MKCQ5599

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S M Yellon et al.
Biology of reproduction, 100(5), 1386-1394 (2019-01-11)
To test the hypothesis that macrophages are essential for remodeling the cervix in preparation for birth, pregnant homozygous CD11b-dtr mice were injected with diphtheria toxin (DT) on days 14 and 16 postbreeding. On day 15 postbreeding, macrophages (F4/80+) were depleted
Biology and molecular epidemiology of diphtheria toxin and the tox gene
The Journal of Infectious Diseases, 181 (2000)
Mary F Fontana et al.
PLoS pathogens, 12(12), e1006046-e1006046 (2016-12-07)
Dynamic regulation of leukocyte population size and activation state is crucial for an effective immune response. In malaria, Plasmodium parasites elicit robust host expansion of macrophages and monocytes, but the underlying mechanisms remain unclear. Here we show that myeloid expansion
Benjamin J Vakoc et al.
Nature medicine, 15(10), 1219-1223 (2009-09-15)
Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability
Vicky A Tobin et al.
Nature, 464(7287), 413-417 (2010-02-26)
Many peptides, when released as chemical messengers within the brain, have powerful influences on complex behaviours. Most strikingly, vasopressin and oxytocin, once thought of as circulating hormones whose actions were confined to peripheral organs, are now known to be released
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service