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D6546

DMEM - high glucose

With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

shipped in

ambient

storage temp.

2-8°C

General description

Listen to an Audio Overview
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.

Other Notes

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D6546-500ML: + D6546-6X500ML: + D6546-24X500ML: + D6546PROC: + D6546-BULK: + D6546-100ML: + D6546-VAR: + D6546-1L:

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