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Merck

D6920

2′-Deoxyadenosine 5′-triphosphate sodium salt solution

10 mM

Synonym(s):

dATP

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About This Item

NACRES:
NA.52
UNSPSC Code:
41106305
EC Number:
217-662-3
Assay:
≥99%
Form:
liquid
Storage temp.:
−20°C

Key Documents

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Quality Level

200

assay

≥99%

form

liquid

concentration

10 mM

technique(s)

PCR: suitable

color

colorless

foreign activity

DNase, RNase, NICKase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

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General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution has been used as a component of the dNTP mix for labelling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe.
2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D6920-.5ML-KC: + D6920-.5ML: + D6920-BULK: + D6920-VAR:

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Spittlebugs (Hemiptera)
Kuznetsova V G, et al.
Protocols for Cytogenetic Mapping of Arthropod Genomes., 351-380 (2015)
Kousuke Sato et al.
Chembiochem : a European journal of chemical biology, 12(15), 2341-2346 (2011-09-03)
We herein describe the synthesis of fluorescent 5-(5,6-dimethoxybenzothiazol-2-yl)-2'-deoxyuridine 5'-triphosphate (d(bt)UTP) and primer extension reactions using d(bt)UTP. We also carried out primer extension reactions using the (bt)U template. B family DNA polymerases, such as KOD, Deep Vent (exo-), and 9°N(m) DNA
Christina M Zimanyi et al.
Structure (London, England : 1993), 20(8), 1374-1383 (2012-06-26)
Ribonucleotide reductases (RNRs) provide the precursors for DNA biosynthesis and repair and are successful targets for anticancer drugs such as clofarabine and gemcitabine. Recently, we reported that dATP inhibits E. coli class Ia RNR by driving formation of RNR subunits
Paul J Rothwell et al.
The Journal of biological chemistry, 288(19), 13575-13591 (2013-03-26)
Conformational selection plays a key role in the polymerase cycle. Klentaq1 exists in conformational equilibrium between three states (open, closed, and “nucleotide-binding”) whose level of occupancy is determined by the bound substrate. The “nucleotide-binding” state plays a pivotal role in
U C Halder et al.
Cell death & disease, 2, e197-e197 (2011-09-02)
During early infection, viruses activate cellular stress-response proteins such as heat-shock proteins (Hsps) to counteract apoptosis, but later on, they modulate these proteins to stimulate apoptosis for efficient viral dissemination. Hsp70 has been attributed to modulate viral entry, transcription, nuclear
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Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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