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D8662

Dulbecco’s Phosphate Buffered Saline

Name: Dulbecco’s Phosphate Buffered Saline
Brand: SIGMA

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32129211

NACRES:

NA.75

MDL number:

MFCD00131855

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Properties

Product Name

Dulbecco′s Phosphate Buffered Saline, With MgCl₂ and CaCl₂, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Application

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.

Dulbecco′s Phosphate Buffered Saline has been used:

as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:

as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
to wash cells for protein isolation

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:

provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
provides a buffering system to maintain the medium within the physiological pH range

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

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Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.