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Merck

D8899

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym(s):

DNA single stranded

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About This Item

CAS Number:
91080-16-9
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
MFCD00163436
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C

Key Documents

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Product Name

Deoxyribonucleic acid, single stranded from calf thymus, lyophilized powder

biological source

bovine (calf) thymus

grade

Molecular Biology

form

lyophilized powder

mol wt

~50 kb

composition

ssDNA, ≥65%

storage temp.

−20°C

Quality Level

200

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Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 25 A260 units.
Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

D8899-BULK-N: + D8899-VAR: + D8899-5X5MG: + D8899-1MG: + D8899-5MG: + D8899-1MG-PW: + D8899-5MG-PW: + D8899-PH: + D8899PROC: + D8899-BULK:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC4481
SLBX9106
SLBV8052
SLBT1595
SLBM6591V

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J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
DNA-cellulose chromatography.
Alberts, B., and Herrick, G.
Methods in Enzymology, 21, 198-217 (1971)
Inessa Halets et al.
International journal of pharmaceutics, 454(1), 1-3 (2013-07-09)
In most articles, cytotoxicity of cationic polyamidoamine (PAMAM) dendrimers toward red blood cells has been exclusively explained by their surface charge. We have focused on dendrimer hydrophobicity as a second possible factor that determines this cytotoxicity. Using PAMAM-NH2 dendrimers from
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