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Merck

F0500

Fast Blue RR Salt

crystalline

Synonym(s):

4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24

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About This Item

Linear Formula:
C15H14ClN3O3 · 1/2 ZnCl2
CAS Number:
14726-29-5
Molecular Weight:
387.89
NACRES:
NA.47
PubChem Substance ID:
24894722
UNSPSC Code:
12171500
EC Number:
238-777-5
MDL number:
MFCD00074762
Colour Index Number:
37155

Key Documents

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Product Name

Fast Blue RR Salt, crystalline

InChI key

LLARVKAOQLUCQI-UHFFFAOYSA-L

InChI

1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4

form

crystalline

technique(s)

microbe id | staining: suitable

solubility

water: 1 mg/mL, clear to hazy, yellow-green

ε (extinction coefficient)

≥15000 at 326-332  nm, ≥7000 at 390-396 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F0500-BULK: + F0500-VAR: + F0500-H: + F0500-5G: + F0500-100G: + F0500-25G:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCN1010
MKCL0751
MKCF5420
MKCC4395
SLBQ8206V

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Advances in Molecular Toxicology (1998)
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Effects of T-lymphocyte depletion on muscle fibrosis in the mdx mouse.
Morrison J, et al.
The American Journal of Pathology, 166, 1701-1701 (2005)
Culling CFA
Handbook of Histopathological and Histochemical Techniques (2013)
Philip F Dobson et al.
Scientific reports, 10(1), 11643-11643 (2020-07-17)
The pathogenesis of declining bone mineral density, a universal feature of ageing, is not fully understood. Somatic mitochondrial DNA (mtDNA) mutations accumulate with age in human tissues and mounting evidence suggests that they may be integral to the ageing process.
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