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Merck

F1141

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75
EC Number:
289-149-2
MDL number:
MFCD00131062
Biological source:
bovine plasma
Form:
solution
Technique(s):
cell culture | mammalian: suitable

Key Documents

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Product Name

Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture

biological source

bovine plasma

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

UniProt accession no.

P07589

binding specificity

Peptide Source: Collagen
, Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Gene Information

bovine ... FN1(280794)

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General description

Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.

Application

Fibronectin (FN) bovine plasma has been used in:
  • fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
  • transwell migration
  • fibronectin assay

Biochem/physiol Actions

Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Other Notes

Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F1141-PH: + F1141PROC: + F1141-VAR: + F1141-BULK: + F1141-1MG: + F1141-1MG-PW: + F1141-5MG: + F1141-2MG-PW: + F1141-5MG-PW: + F1141-2MG: + F1141-BULK-N:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG8374
SLCD2908
SLBZ7025
SLBZ5423
SLBX5503

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Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
Khang D, et al.
Biomaterials, 33(26), 5997-6007 (2012)
Mary Cathleen McKinney et al.
BMC biology, 14(1), 111-111 (2016-12-17)
Collective neural crest cell migration is critical to the form and function of the vertebrate face and neck, distributing bone, cartilage, and nerve cells into peripheral targets that are intimately linked with head vasculature. The vasculature and neural crest structures
View All Related Papers

Articles

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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