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Merck

F3013

Ferredoxin from Spinacia oleracea (spinach)

lyophilized powder, Ferredoxin ≥15 wt. %

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About This Item

CAS Number:
9040-09-9
UNSPSC Code:
12352202
NACRES:
NA.61
MDL number:
MFCD00131049
Form:
lyophilized powder
Biological source:
spinach (Spinacia oleracea)

Key Documents

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biological source

spinach (Spinacia oleracea)

Quality Level

200

form

lyophilized powder

composition

Ferredoxin, ≥15 wt. %

solubility

water: 2 mg/mL, clear, faintly brown

application(s)

cell analysis

storage temp.

−20°C

Gene Information

spinach ... SOVF(137530)

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General description

Ferredoxin I (Fd I) from Spinacia oleracea comprises 97 amino acid and is an iron sulfur cluster (2Fe-2S). It is synthesized in the chloroplast. The structure of Fd I has conserved active site.

Application

Ferredoxin from Spinacia oleracea (spinach) has been used:
  • as a substrate for spinach ferredoxin reductase,
  • for reconstitution with chlorophyll-A for scanning electron microscope (SEM) dispersive X-ray analyzer (EDX) integrated analysis,
  • as a reductant in adenylylsulfate (APS) reductase activity of P. aeruginosa

Biochem/physiol Actions

Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation. It has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium.
Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Packaging

Package sizes are based on ferredoxin content.

Physical form

Contains Tris buffer

Preparation Note

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

F3013PROC: + F3013-5MG-PW: + F3013-BULK-N: + F3013-1MG: + F3013-BULK: + F3013-5MG: + F3013-10MG-PW: + F3013-VAR: + F3013-1MG-PW: + F3013-10MG:

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Certificates of Analysis (COA)

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Extraction of ferredoxin from spinach leaves and its effect on Clostridium perfringens (anaerobe) growth
Osman ZA, et al.
European journal of molecular biotechnology, 3(4), 267-285 (2013)
Identification of a new class of 5?-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria
Bick JA, et al.
Journal of Bacteriology, 182(1), 135-142 (2000)
Structure of the Mutant E92K of [2Fe-2S] Ferredoxin I from Spinacia oleracea at 1.7 AA Resolution
Binda C, et al
Acta Crystallographica Section D, Biological Crystallography, 54(6), 1353-1358 (1998)
Keita Miyake et al.
The FEBS journal, 287(18), 4016-4031 (2020-01-30)
Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing
Harnessing a P450 fatty acid decarboxylase from Macrococcus caseolyticus for microbial biosynthesis of odd chain terminal alkenes
Lee JW, et al.
Metabolic Engineering Communications, 7, e00076-e00076 (2018)
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Protocols

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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