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F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
NACRES:
NA.47
PubChem Substance ID:
24894860
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
MFCD00005063
Beilstein/REAXYS Number:
1407295
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Product Name

Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

Quality Level

100

assay

≥97.5% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to very dark yellow

mp

>360 °C (lit.)

solubility

acetone: soluble 10 mg/mL

fluorescence

λex 492 nm; λem 518 nm (green)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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