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Merck

F6765

Fetal Bovine Serum

USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00132239
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice

Key Documents

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Product Name

Fetal Bovine Serum, USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Select

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

shipped in

dry ice

storage temp.

−20°C

Quality Level

500

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Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Charcoal stripped FBS is often used instead of FCS to minimize the level of androgen and other hormones provided in 10% serum supplemented media.

Biochem/physiol Actions

Charcoal Stripped Fetal Bovine Serum (FBS) is available for researchers requiring decreased levels of various hormones in their studies. Dextran treated Charcoal is used to selectively remove hormones without nonspecific loss of other serum components. The effectiveness of hormone removal is assessed on the basis of reductions in the levels of T3, T4, and Progesterone pre and post treatment.

This product is designed for researchers involved in studying processes influenced by steroid hormones such as estrogen stimulation and the obesity process and other areas that benefit from reduced hormone levels such as certain types of viral infections.

Other Notes

The product is not available in Australia. Please view our protocol for Charcoal-stripping FBS to deplete hormones here.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
20H022
19M018
16M289
16C075
12H047

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Ahmad Sharanek et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(12), 1292-1303 (2017-09-21)
Drug-induced intrahepatic cholestasis is characterized by cellular accumulation of bile acids (BAs), whose mechanisms remain poorly understood. The present study aimed to analyze early and progressive alterations of BA profiles induced by cyclosporine A, chlorpromazine, troglitazone, tolcapone, trovafloxacin, and tacrolimus
Christian D. Hernandez-Silva et al.
Anti-cancer agents in medicinal chemistry, 19(6), 783-791 (2019-02-08)
The effect of estrogen has been traditionally studied through the modulation of its alpha and beta nuclear receptors; however, the G Protein-Coupled Estrogen Receptor (GPER) has been recently involved in the pathology of numerous tumors. Although the study of GPER
Jonathan T Lei et al.
Cell reports, 24(6), 1434-1444 (2018-08-09)
RNA sequencing (RNA-seq) detects estrogen receptor alpha gene (ESR1) fusion transcripts in estrogen receptor-positive (ER+) breast cancer, but their role in disease pathogenesis remains unclear. We examined multiple ESR1 fusions and found that two, both identified in advanced endocrine treatment-resistant
Anna K Wójtowicz et al.
Neurotoxicity research, 35(1), 183-195 (2018-08-19)
Di-(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in various plastic compounds, such as polyvinyl chloride (PVC), and products including baby toys, packaging films and sheets, medical tubing, and blood storage bags. Epidemiological data suggest that phthalates increase the risk
Yusuke Ohnishi et al.
PloS one, 3(5), e2248-e2248 (2008-05-22)
Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi), the design and assessment of small interfering RNA
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Articles

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

細胞および組織培養用血清に関するFAQ

FBSおよびその他の血清製品に関するよくあるお問い合わせ（FAQ）への回答および関連製品のリンクはこちら。

ウシ胎児血清（FBS）– 知っておくべきこと

細胞培養にウシ胎児（または子ウシ）血清を使用する際の基本と、調達や規制が細胞アッセイ用血清の価格にどのように影響するのかご確認ください。

Protocols

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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用途に適したウシ胎児血清

メルクのウシ胎児血清（FBS）のポートフォリオから、お客様のニーズとご予算に合う最適なFBSをお探しください。血清サンプルのご依頼。

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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