F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Synonym(s):
FITC, Fluorescein 5-isothiocyanate
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About This Item
Product Name
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
assay
≥90% (HPLC)
form
powder
technique(s)
titration: suitable
color
orange to dark orange
mp
>360 °C (lit.)
solubility
acetone: 1 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
suitability
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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Application
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
General description
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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