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Merck

F7378

Fluorescein diacetate

Powder

Synonym(s):

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
596-09-8
Molecular Weight:
416.38
NACRES:
NA.47
PubChem Substance ID:
24894939
UNSPSC Code:
12171500
EC Number:
209-877-6
MDL number:
MFCD00005062
Beilstein/REAXYS Number:
62575

Key Documents

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Product Name

Fluorescein diacetate, used as cell viability stain

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

form

powder

technique(s)

titration: suitable

color

white to faint yellow

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.
Lipase substrate

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCM9758
MKCC8346
MKBR3002V
MKBF6522V
43396KJV

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Effect of shock waves on suspended and immobilized L1210 cells.
Brummer F
Ultrasound in Medicine & Biology, 15, 229-229 (1989)
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Zhang J
Biomedical Materials (Bristol, England), 9, 035011-035011 (2014)
Cell Growth, Differentiation and Senescence: A Practical Approach (1999)
Design of multifunctional micelle for tumor-targeted intracellular drug release and fluorescent imaging.
Weiwei Wang et al.
Advanced materials (Deerfield Beach, Fla.), 24(1), 115-120 (2011-12-07)
Koichi Baba et al.
Journal of controlled release : official journal of the Controlled Release Society, 153(3), 278-287 (2011-05-10)
This report describes a method for enhancing the ocular penetration of eye drops using nanoparticles of hydrolyzable dye, which is similar to a prodrug approach. The entry of eye drops into the ocular globe is restricted predominantly by corneal barrier
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