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Merck

FBS25

Fast Blue RR Salt

Preweighed Capsules

Synonym(s):

4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24

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About This Item

Linear Formula:
C15H14ClN3O3 · 1/2 ZnCl2
CAS Number:
14726-29-5
Molecular Weight:
387.89
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24895004
EC Number:
238-777-5
Colour Index Number:
37155
MDL number:
MFCD00074762

Key Documents

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InChI key

LLARVKAOQLUCQI-UHFFFAOYSA-L

InChI

1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4

form

powder

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

dry ice

storage temp.

−20°C

Quality Level

500

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Related Categories

Application

Used as a kit component for the determination of alkaline phosphatase in Sigma Kits 85L1 and 85L2 and for the determination of nonspecific esterase in Sigma Kit 90A1.
Fast Blue RR Salt has been used for the staining of alkaline phosphatase activity in cells.

General description

Fast Blue RR is part of the azo coupling system and is required for the staining of hydrolytic enzymes. In the presence of naphthol, it results in the formation of an insoluble coloured precipitate at the site of enzyme activity.

Packaging

Actual weight of the capsule will vary with dye lot and has been optimized by assay.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FBS25-10CAP-KC: + FBS25-VAR: + FBS25-10CAP: + FBS25-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL1605
SLCD6230
SLBX9842
SLBT8315
SLBS4561

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Manchenko GP
Handbook of Detection of Enzymes on Electrophoretic Gels (2002)
Emergence of patterned stem cell differentiation within multicellular structures.
Ruiz SA and Chen CS
Stem Cells, 26, 2921-2921 (2008)
Jung-Min Kim et al.
Nature communications, 11(1), 2289-2289 (2020-05-10)
The osteoblast differentiation capacity of skeletal stem cells (SSCs) must be tightly regulated, as inadequate bone formation results in low bone mass and skeletal fragility, and over-exuberant osteogenesis results in heterotopic ossification (HO) of soft tissues. RUNX2 is essential for
Argiris Papathanasopoulos et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 29(9), 1327-1335 (2011-03-25)
An unexpected side effect of some classes of anticoagulants has been osteoporosis which may be, at least in part, related to deranged mesenchymal stem cell (MSC) function. The aim of the present study was to compare the effect of fondaparinux

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