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Merck

G1041

EZBlue™ Gel Staining Reagent

Synonym(s):

protein gel stain, protein stain

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About This Item

NACRES:
NA.32
UNSPSC Code:
41105300

Key Documents

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technique(s)

protein staining: suitable

Quality Level

200

storage temp.

2-8°C

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General description

Convenient, sensitive, and safe, EZBlue™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Application

EZBlue™ Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Features and Benefits

  • Premixed solution eliminates the time and effort required to prepare the stain
  • Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
  • Rapid reaction significantly reduces the amount of time required to stain and rinse
  • No solvent waste so you save time and money by eliminating hazardous material disposal

Other Notes

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

Legal Information

EZBlue is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Storage Class

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

G1041-500ML:4.548174006795E12

jan

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Certificates of Analysis (COA)

Lot/Batch Number
078K4357
058K4362
087K4349
027K4348
036K4369

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Rakhi Panda et al.
Journal of agricultural and food chemistry, 63(43), 9629-9639 (2015-10-09)
Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this
Antibodies to the GABA(B) receptor in limbic encephalitis with seizures: case series and characterisation of the antigen.
Lancaster E, et al.
Lancet Neurology, 9, 67-76 (2010)
AMPA receptor antibodies in limbic encephalitis alter synaptic receptor location.
Lai M, et al.
Annals of Neurology, 65, 424-434 (2009)
Praveen Maurye et al.
Electrophoresis, 38(16), 2060-2068 (2017-04-27)
PAGE is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and
Nathan L Marsteller et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 141, 111398-111398 (2020-05-22)
Currently no validated animal model is predictive of human responses in ranking purified dietary proteins in the prevalence or potency of food allergy in humans. Since the gastrointestinal microbiota is thought to influence oral tolerance, we hypothesize that a germ-free
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Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Protocols

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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メルクのプレミックス溶液とプロトコルでSDS-PAGEゲル調製を簡素化し、正確なタンパク質分離を達成しましょう。

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