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Merck

H0137

HT Media Supplement (50×) Hybri-Max™

lyophilized powder, suitable for hybridoma, BioXtra

Synonym(s):

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Product Name

HT Media Supplement (50×) Hybri-Max™, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

Quality Level

200

Related Categories

Application

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Preparation Note

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

H0137-1VL-PW: + H0137-BULK: + H0137-1VL-KC: + H0137-1VL-IR: + H0137-10VL-PW: + H0137-PH: + H0137PROC: + H0137-10VL: + H0137-VAR: + H0137-1VL:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK2065
SLCK0428
SLCJ8247
SLCJ6851
SLCJ5645

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Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii
Gondim L, et al.
Experimental Parasitology, 163, 46-56 (2016)
Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell
Jiao A, et al.
International Journal of Endocrinology, 2018 (2018)
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress
Hashiguchi K, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 808, 10-19 (2018)
Kimberly A McCormack et al.
Parasitology research, 118(8), 2409-2417 (2019-06-15)
Human babesiosis, a tick-borne disease similar to malaria, is most often caused by the hemoprotozoans Babesia divergens in Europe, and Babesia microti and Babesia duncani in North America. Babesia microti is the best documented and causes more cases of human
Emmanuel Osei Mensah et al.
Journal of bioscience and bioengineering, 128(4), 487-494 (2019-04-30)
Since the use of protein therapeutics is effective for treating intractable human diseases, the production of biologic therapeutic agents has dramatically increased over the past three decades. The Chinese hamster ovary (CHO) cell lines are the most commonly used host
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Protocol Guide: XTT Assay for Cell Viability and Proliferation

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細胞生存率と細胞増殖のMTTアッセイプロトコル

細胞生存率、細胞増殖、および細胞毒性測定用のMTTアッセイプロトコル。MTT試薬の調製手順と応用例。

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96ウェルマイクロプレートで培養した接着／浮遊細胞の細胞増殖、生存率、細胞毒性の非放射性定量のための比色分析法を実施できます。

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モノクローナル抗体の製造と精製

大きな技術的進歩により、モノクローナル抗体のより迅速で効率的な製造が実現しました。抗体発見のために確立されたプラットフォームは3つあります。メルクは、それぞれの方法を使ったモノクローナル抗体ライブラリー製造のための試薬をご提供しています。

Product Information Sheet - H0137

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