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Merck

H1029

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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About This Item

NACRES:
NA.56
UNSPSC Code:
12352203

Key Documents

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Product Name

Monoclonal Anti-polyHistidine antibody produced in mouse, clone HIS-1, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

liquid

technique(s)

dot blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Application

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.

Biochem/physiol Actions

The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

Immunogen

recombinant polyHistidine tagged fusion protein.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Physical form

Suppled as ascites fluid with 15mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
079M4822V
098M4845V
097M4894V
106M4768V
033M4785

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G protein-coupled receptor-promoted trafficking of G?1?2 leads to AKT activation at endosomes via a mechanism mediated by G?1?2-Rab11a interaction.
Garcia-Regalado A, et al.
Molecular Biology of the Cell, 19(10), 4188-4200 (2008)
Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Savitsky D A, et al.
Proceedings of the National Academy of Sciences of the USA (2010)
Elise Pinta et al.
The Journal of biological chemistry, 285(36), 28333-28342 (2010-07-03)
Yersinia enterocolitica (Ye) is a gram-negative bacterium; Ye serotype O:3 expresses lipopolysaccharide (LPS) with a hexasaccharide branch known as the outer core (OC). The OC is important for the resistance of the bacterium to cationic antimicrobial peptides and also functions
Association of a novel pre-ribosomal complex in Trypanosoma brucei.
Wang L
Eukaryotic Cell (2013)
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