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Merck

HT501320

Formalin solution, neutral buffered, 10%

histological tissue fixative

Synonym(s):

10% Neutral Buffered Formalin, 10% NBF, Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
24895889
NACRES:
NA.47
MDL number:
MFCD00003274

Key Documents

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SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

biological source

synthetic

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

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Application

Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
  • It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
  • It is used for routine histological staining, providing clear and detailed images of cellular structures.
Popular general purpose histological fixative.

Biochem/physiol Actions

Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.

Features and Benefits

  • 10% NBF is well-suited for large throughput laboratories.
  • It requires a relatively short period of fixation.
  • It can be used for the long-term storage of tissue.

General description

10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Packaging

Item is packaged in a 10L Cubitainer consisting of a plastic bladder inside of a cardboard box with an attached spigot.

Physical form

Neutral buffered solution

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

wgk

WGK 3

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

Specified Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

HT501320-VAR: + HT501320-9.5L-H: + HT501320-9.5L-PW: + HT501320-BULK: + HT501320-9.5L:4548173291765

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ2804
MKCM6879
MKCL5217
MKCK6772
MKCK6906

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