Skip to Content
Merck

I8896

IGEPAL® CA-630

Molecular Biology

Synonym(s):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
UNSPSC Code:
12352104
NACRES:
NA.31

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

IGEPAL® CA-630, Molecular Biology

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

grade

Molecular Biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

mp

6 °C

solubility

water: soluble at 20 °C

Quality Level

200

viscosity

~250-350 cP(25 °C)

density

1.060 g/cm3

HLB

13

foreign activity

DNase and RNase, none detected

Looking for similar products? Visit Product Comparison Guide

Related Categories

Analysis Note

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Application

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

Disclaimer

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Other Notes

Nonionic, non-denaturing detergent

Legal Information

IGEPAL is a registered trademark of Solvay

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCQ0864
MKCN5898
MKCM9178
MKCM3369
MKCL4156

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Soichiro Yamanaka et al.
Developmental cell, 51(1), 21-34 (2019-09-03)
Facultative heterochromatin forms and reorganizes in response to external stimuli. However, how the initial establishment of such a chromatin state is regulated in cell-cycle-arrested cells remains unexplored. Mouse gonocytes are arrested male germ cells, at which stage the genome-wide DNA
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
View All Related Papers

Protocols

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Related Content

タンパク質プルダウンテクニック

親和性、GSTプルダウン、TAP、共免疫沈降法を利用して、プルダウンアッセイでin vitroタンパク質間相互作用を調査します。

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service