Key Documents

View All Documentation

L2542

LB Broth (Miller)

Name: LB Broth (Miller)
Brand: SIGMA

Liquid microbial growth medium

Synonym(s):

Luria Broth Miller, Luria-Bertani broth, Miller

Select a Size

Change View

About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

grade

Molecular Biology

Quality Level

400

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
selective cultivation when appropriate antibiotics are added.
the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:

Filter-sterilized, ready-to-use format
Convenient package size
Standard formulation

Other Notes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Still not finding the right product?

Explore all of our products under LB Broth (Miller)

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Articles

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

タンパク質発現系

遺伝子工学により、研究のための遺伝子組換えタンパク質の大規模な発現・単離が可能になります。

Sialylation of group B streptococcal capsular polysaccharide is mediated by cpsK and is required for optimal capsule polymerization and expression.

D O Chaffin et al.

Journal of bacteriology, 187(13), 4615-4626 (2005-06-22)

Several bacterial pathogens have evolved the means to escape immune detection by mimicking host cell surface carbohydrates that are crucial for self/non-self recognition. Sialic acid, a terminal residue on these carbohydrates, inhibits activation of the alternate pathway of complement by

Weighing of biomolecules, single cells and single nanoparticles in fluid.

Thomas P Burg et al.

Nature, 446(7139), 1066-1069 (2007-04-27)

Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires

Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50

Antonio Basanta, Beatriz Gomez-Sala, et al.

Biotechnology, 76, 3314-3324 (2010)

View All Related Papers