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Merck

L6544

LuminoCt® SYBR® Green qPCR ReadyMix™

For fast SYBR Green quantitative PCR

Synonym(s):

LuminoCt®

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About This Item

NACRES:
NA.55
UNSPSC Code:
41106300

Key Documents

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form

liquid

usage

sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions

feature

dNTPs included, hotstart

Quality Level

200

technique(s)

RT-qPCR: suitable, qPCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

compatibility

Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Opticon, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.
  • Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Application

LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.

Features and Benefits

  • Assay results in as little as 25 minutes
  • Unsurpassed accuracy, precision, and sensitivity
  • Virtually eliminates the need to optimize assay parameters
  • Ideal for performing high-throughput, quantitative PCR applications
  • The master mix allows consistency from one reaction to the next
  • Reduced preparation time and reduced risk of contamination from multiple pipetting steps
  • The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
  • This master mix is compatible with commercial primer sets, including TaqMan® assays

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

  • At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
  • Sigma recommends designing primers and probes with Beacon Designer software
  • To order primers and probes from Sigma, visit: www.sigma.com/oligos

Legal Information

Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Only

Product No.
Description
  • 2 X

Kit Components Also Available Separately

Product No.
Description
SDS
  • R4526100X ROX internal reference dyeSDS

Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L6544-500RXN: + L6544-BULK: + L6544-VAR: + L6544-100RXN: + L6544-2000RXN:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK8231
SLCJ2171
SLCH0542
SLCD2378
SLBZ2473

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Khrystyna Levytska et al.
PPAR research, 2014, 637251-637251 (2014-04-09)
Common pregnancy complications, such as severe preeclampsia and intrauterine growth restriction, disrupt pregnancy progression and impair maternal and fetal wellbeing. Placentas from such pregnancies exhibit lesions principally within the syncytiotrophoblast (SCT), a layer in direct contact with maternal blood. In
Devika Sharanya et al.
Evolution & development, 17(1), 34-48 (2015-01-30)
Studies of vulval development in the nematode C. elegans have identified many genes that are involved in cell division and differentiation processes. Some of these encode components of conserved signal transduction pathways mediated by EGF, Notch, and Wnt. To understand
Jan Gravemeyer et al.
Oncogene, 41(1), 37-45 (2021-10-21)
Merkel cell carcinoma (MCC) is a neuroendocrine tumor either induced by integration of the Merkel cell polyomavirus into the cell genome or by accumulation of UV-light-associated mutations (VP-MCC and UV-MCC). Whether VP- and UV-MCC have the same or different cellular
Oksana Shynlova et al.
Journal of cellular and molecular medicine, 17(2), 311-324 (2013-02-06)
Leucocyte infiltration in the decidua (maternal-foetal interface) before, during and after term (TL) and preterm labour (PTL) was studied in mouse. We also investigated the mechanism of peripheral leucocyte recruitment into decidua by analysing the tissue cytokine profiles. Decidual tissues
Oksana Shynlova et al.
Journal of cellular and molecular medicine, 17(1), 90-102 (2012-12-05)
This study aimed to determine the mechanism of uterine activation during labour, both term (TL) and preterm (PTL). We hypothesized that the peripheral leucocytes are recruited to uterine tissues by locally produced cytokines where they contribute to the initiation of
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Articles

PCRマスターミックス

PCRマスターミックスは、DNAポリメラーゼ、dNTP、MgCl2、バッファーのような成分によりPCR/RT-PCRの反応を簡略化するもので、市販のものも自身で作製するものもあります。

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Protocols

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Related Content

Product Information Sheet - L6544
蛍光シリカナノ粒子のバイオコンジュゲーション

バイオマテリアルの蛍光シリカナノ粒子への受動的な吸着と共有結合による結合方法をご紹介します。

RT-qPCR – 定量的逆転写PCR

RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。

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