Skip to Content
Merck

L9393

Anti-Laminin (LAMA1) antibody

rabbit polyclonal

Synonym(s):

Laminin Antibody

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162586
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, DB, IHC (p)
Citations:
1189

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-Laminin antibody produced in rabbit, 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

animal, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

0.5 mg/mL

technique(s)

dot blot: 1:1,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues, microarray: suitable

UniProt accession no.

P25391

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

300

Gene Information

human ... LAMA1(284217)
mouse ... Lama1(16772)

Related Categories

General description

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.

Immunogen

Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

Application

Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.
Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.

Biochem/physiol Actions

LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.
Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

L9393-.2ML: + L9393-.5ML: + L9393-VAR: + L9393-100UL: + L9393-BULK:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
099M4886V
21190515
069M4885V
029M4802V
21181054

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Autoimmunity against laminins.
Florea F, et al.
Clinical Immunology (Orlando, Fla.), 39-52 (2016)
Mutations in LAMA1 Cause Cerebellar Dysplasia and Cysts with and without Retinal Dystrophy
Kimberly AA, et al.
American Journal of Human Genetics, 95(2), 227-234 (2014)
Barbara Vincent et al.
Journal of applied physiology (Bethesda, Md. : 1985), 109(2), 564-573 (2010-05-29)
The ACTN3 gene encodes for the alpha-actinin-3 protein, which has an important structural function in the Z line of the sarcomere in fast muscle fibers. A premature stop codon (R577X) polymorphism in the ACTN3 gene causes a complete loss of
Yongchol Shin et al.
American journal of physiology. Cell physiology, 317(2), C314-C325 (2019-06-13)
Angiogenesis is a process through which new blood vessels are formed by sprouting and elongating from existing blood vessels. Several methods have been used to replicate angiogenesis in vitro, including culturing vascular endothelial cells on Matrigel and coculturing with endothelial
Solving an enigma: Arterial pole development in the zebrafish heart
Adrian CG, et al.
Developmental Biology, 290(2), 265-276 (2006)
View All Related Papers

Related Content

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Data Sheet - L9393

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service