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M3942

DMEM - high glucose

MegaCell™, with sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell cutlure

Synonym(s):

DMEM, DMEM Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

MegaCell™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

Quality Segment

300

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

General description

MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Application

MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
  • as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
  • as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
  • to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Preparation Note

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

M3942-BULK: + M3942-500ML: + M3942-VAR: + M3942-PH: + M3942PROC:

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