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Merck

M5017

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

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Product Name

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

M5017-50K: + M5017-ALC: + M5017-2X5L: + M5017-10X1L: + M5017-VAR: + M5017-ALC2: + M5017-10L: + M5017-50KG: + M5017-1L: + M5017LF1-BIBC: + M5017-BULK: + M5017-BIBC: + M5017-50L: + M5017-25K: + M5017-PROC: + M5017-25KG:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBX3254
SLBW8761
SLBW8690
SLBT6440
SLBT5523

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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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