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M8403

McCoy’s 5A Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

5A basal medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
EC Number:
200-655-4
MDL number:
MFCD00217560
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Product Name

McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

shipped in

ambient

storage temp.

2-8°C

General description

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used to maintain:

  • G-361 melanoma cells;
  • 87-MG cells
  • human colon carcinoma cell line HCT116

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Supplement with 0.22 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

M8403-BULK: + M8403PROC: + M8403-24X500ML: + M8403-100ML: + M8403-PH: + M8403-500ML: + M8403-6X500ML: + M8403-VAR:

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