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Merck

MAK052

ALT Activity Assay

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Alanine Aminotransferase Activity Assay Kit, SGPT Activity Assay Kit, Serum Glutamic-Pyruvic Transaminase Activity Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

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usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric, fluorometric

relevant disease(s)

gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)

General description

Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.

Application

Suitable for the measurement of alanine aminotransferase (ALT) activity in biological samples including tissue, cells and serum.
ALT Activity Assay kit has been used to determine the activity of alanine aminotransferase (ALT) in serum samples.

Biochem/physiol Actions

The ALT Activity Assay Kit provides a simple and direct procedure for measuring ALT activity in a variety of biological samples.

ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 μmole of pyruvate per minute at 37 °C.

Features and Benefits

Compatible with high-throughput handling systems.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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fsl

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ishl_indicated

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