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Merck

MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

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usage

sufficient for 200 colorimetric or fluorometric tests

species reactivity

Drosophila, human, mouse

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

accession no.

NM_000167.6, NM_001273184.2, P13706, P32189

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

input

food(s)
beverage(s)
biological sample(s)

Gene Information

Drosophila ... Gpdh1(33824)
human ... GK(2710 )
mouse ... Gpd1(14555)

Related Categories

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Biochem/physiol Actions

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Features and Benefits

Compatible with high-throughput handling systems.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Please refer to KIT Component information

pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

Lot/Batch Number
117CB10A08
117CB06A23
117CB05A11
117CB03A11
117CB03A02

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Nicolae S.
Health & Drugs: Disease, Prescription & Medication (2014)
Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
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