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Merck

MAK122

Phospholipid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Phospholipid Quantification Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

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usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages
pharmaceutical

detection method

colorimetric, fluorometric

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Application

Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.
Suitable for the detection of phospholipids in biological samples

Biochem/physiol Actions

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

Features and Benefits

Compatible with high-throughput handling systems.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334,H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Certificates of Analysis (COA)

Lot/Batch Number
122CC01A27
122CB11A03
122CB07A29
122CB03A17
122CA10A12

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