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N2640

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24897580

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

Beilstein/REAXYS Number:

5324661

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Properties

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

form

tablet

Quality Level

200

solubility

water: 1 tablet/3 mL, clear, colorless

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

Preparation Note

Contains 15 mg substrate per tablet

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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