NUC101
Nuclei Isolation Kit: Nuclei EZ Prep
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Synonym(s):
Rapid mammalian nuclei isolation kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
usage
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Quality Segment
shelf life
1 yr at 2‑8 °C
packaging
pkg of 1 kit
application(s)
cell analysis
foreign activity
nuclease and protease, free
shipped in
wet ice
storage temp.
2-8°C
General description
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.
Application
Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
Biochem/physiol Actions
The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
Other Notes
All procedures should be carried out on ice or 2-8°C.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
Please refer to KIT Component information
pdsc
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prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
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