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Merck

O1516

Oil Red O solution

0.5% in propylene glycol

Synonym(s):

Solvent Red 27, Sudan Red 5B

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About This Item

Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
1320-06-5
Molecular Weight:
408.49
UNSPSC Code:
12171500
NACRES:
NA.47

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SMILES string

N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

form

liquid

concentration

0.5% in propylene glycol

technique(s)

microbe id | staining: suitable

λmax

0.40-1.00 at 506-520 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

217.4 °F

flash_point_c

103 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water insoluble liquid

fsl

O1516-VAR: + O1516-BULK: + O1516-500ML: + O1516-250ML:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH2016
SLCF8267
SLCB3640
SLBX9267
SLBW8813

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