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Merck

P1995

Anti-Podoplanin (HG-19) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD05665247
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Species reactivity:
rat
Citations:
14
Technique(s):
indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections, western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung
Uniprot accession no.:
Q64294

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~38 kDa

species reactivity

rat

technique(s)

indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections, western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung

UniProt accession no.

Q64294

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Pdpn(54320)

Application

Anti-Podoplanin (HG-19) antibody produced in rabbit has been used in immunostaining, immunoblotting and immunofluorescence staining.

Biochem/physiol Actions

Podoplanin expression is regulated by the lymphatic-specific homeobox gene Prox-1. Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It has a critical regulatory role in the formation of the lymphatic vasculature. Podoplanin does not seem to be upregulated in tumor angiogenesis. It is detectable in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas.
Podoplanin is a marker of lymphatic endothelial cells. It may have a role in tumor cell invasion and migration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Podoplanin is part of the type-1 transmembrane sialomucin-like glycoprotein family. The gene encoding this 43kDa protein is localized on rat chromosome 5.
Podoplanin is present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is also named E11 antigen and has been described to be present in normal, inflammatory, and neoplastic lymphatic capillary endothelium. Podoplanin is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells. It is composed of heavily glycosylated extracellular domain and a short intracytoplasmic tail.

Immunogen

synthetic peptide of rat podoplanin (amino acids 111-129) with N-terminal cysteine, conjugated to KLH. The sequence in mouse differs by one residue.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P1995-200UL: + P1995-BULK: + P1995-VAR: + IXO11490:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
113M4832
123K4887

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Selective Loss of Podoplanin Protein Expression Accompanies Proteinuria and Precedes Alterations in Podocyte Morphology in a Spontaneous Proteinuric Rat Model
Klaas Koop
The American Journal of Pathology (2008)
Podoplanin is a useful diagnostic marker for epithelioid hemangioendothelioma of the liver
Fujii T, et al.
Modern Pathology, 21(2), 125-125 (2007)
Glomerular hypertrophy precedes albuminuria and segmental loss of podoplanin in podocytes in Munich-Wistar-Fromter rats
D.H.T.Ijelaar
The American Journal of Physiology (2007)
Renoprotective effects of telmisartan in the 5/6
nephrectomised rats
Ichiro Tsunenari
Journal of the renin-angiotensin-aldosterone system : JRAAS (2007)
Methylglyoxal Induced Basophilic Spindle Cells with Podoplanin at the Surface of Peritoneum in Rat Peritoneal Dialysis Model
Ichiro Hirahara
BioMed Research International (2015)
View All Related Papers

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