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Merck

P4557

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology

Synonym(s):

Tris-equilabrated Phenol

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About This Item

Empirical Formula (Hill Notation):
C6H6O
CAS Number:
108-95-2
Molecular Weight:
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
57654529
EC Number:
203-632-7
Beilstein/REAXYS Number:
6428463
MDL number:
MFCD00002143
Grade:
Molecular Biology
Assay:
≥99.0% (GC)

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Product Name

Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

SMILES string

Oc1ccccc1

grade

Molecular Biology

product line

BioReagent

assay

≥99.0% (GC)

form

liquid

dilution

(for analytical testing)

pH

7.7-8.1 (25 °C)

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... GABRA1(2554)

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Application

Phenol solution has been used:

  • as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
  • for extraction of DNA from Arabidopsis thaliana Rosette leaves
  • in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
  • as a component of extraction buffer to extract RNA from fruit pulp
  • for the extraction of protein from roots
  • for the isolation of fecal DNA
  • for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Other Notes

  • 1 bottle Tris-equilabrated Phenol (product number P4557)
  • Equilibration Buffer (product B5658)

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ8367
MKCN2868
MKCN2870
MKCN2866
MKCM0215

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Michael B Powner et al.
Nature protocols, 7(6), 1086-1096 (2012-05-12)
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International Archives of Allergy and Immunology, 175(1-2), 77-84 (2018)
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Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
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Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
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