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P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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About This Item

CAS Number:
37288-19-0
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
MFCD00131896
EC Number:
4.6.1.13 (BRENDA, IUBMB)
Specific activity:
≥1,000 units/mg protein (Lowry)
Biological source:
Bacillus sp. (Bacillus cereus)
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biological source

Bacillus sp. (Bacillus cereus)

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

≥1,000 units/mg protein (Lowry)

mol wt

28 kDa

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

storage temp.

2-8°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:
  • in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
  • to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
  • to cleave immunolabeled HeLa cells

Biochem/physiol Actions

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.
Used for the release of GPI anchored proteins from the membrane.

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

P8804-25UN-PW: + P8804-BULK: + P8804-VAR: + P8804-25UN: + P8804-5UN: + P8804-5UN-PW:

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