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Merck

P9187

OPD

SIGMAFAST™, peroxidase substrate, chromogenic, tablet

Synonym(s):

OPD Peroxidase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

SIGMAFAST™ OPD, tablet

form

tablet

solubility

water: 0.5 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

Related Categories

Application

SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.

Features and Benefits

SIGMAFAST™ OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

Preparation Note

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

This substrate is hygroscopic and light sensitive

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

P9187-BULK: + P9187-VAR: + P9187-50SET: + P9187-5SET:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ3691
SLCG4913
SLCC0308
SLBZ5219
SLBS6448

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Subcutaneous absorption of monoclonal antibodies: role of dose, site of injection, and injection volume on rituximab pharmacokinetics in rats
Kagan L, et al.
Pharmaceutical Research, 29(2), 490-499 (2012)
Yoo Min Park et al.
Nano convergence, 7(1), 29-29 (2020-09-02)
The noninvasive early detection of biomarkers for Alzheimer's disease (AD) is essential for the development of specific treatment strategies. This paper proposes an advanced method for fabricating highly ordered and flexible nanopillar-based electrochemical biosensors by the combination of soft/photolithography and
Eliana Spilioti et al.
Environmental geochemistry and health, 39(4), 821-833 (2016-07-23)
Spa resorts are known for thousands of years for their healing properties and have been empirically used for the treatment of many inflammatory conditions. Mud is one of the most often used natural materials for preventive, healing and cosmetic reasons
Gisela Esquerda-Canals et al.
PloS one, 14(5), e0217793-e0217793 (2019-06-01)
The success of the targeting of amyloid-β (Aβ) oligomers through immunotherapy in Alzheimer's disease (AD) mouse models has not been translated into the clinics. The use of single-chain variable fragments (scFvs) has been proposed to prevent the potential severe effects
Elisa Lobato et al.
Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
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Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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