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R1780

RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, suitable for isotope labeling for cell culture applications

Synonym(s):

RPMI 1640 Cell Culture Medium, Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

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Properties

Product Name

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-¹³C₆, ¹⁵N₄ HCl (Product 608033), 0.100 g/L L-Leucine-¹³C₆, ¹⁵N (Product 608068), and 0.100 g/L L-Lysine-¹³C₆, ¹⁵N₂ (Product 608041).

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R1780-PH: + R1780PROC: + R1780-VAR: + R1780-500ML: + R1780-BULK: + R1780-1G:

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Certificates of Analysis (COA)

Lot/Batch Number

019K8418

108K8405

127K8406

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