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Merck

R4775

REDExtract-N-Amp™ PCR ReadyMix™

Ready-to-use 2X PCR Master Mix with Loading Dye

Synonym(s):

Direct plant PCR master mix, Direct tissue PCR master mix, PCR master mix with loading dye

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About This Item

NACRES:
NA.55
UNSPSC Code:
41106303

Key Documents

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usage

sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications

feature

dNTPs included, hotstart

color

red

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

General description

The REDExtract-N-Amp™ PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.

Application

REDExtract-N-Amp™ PCR Master Mix has been used:
  • for the genotyping of fixed zebrafish embryo samples
  • in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp™ Plant PCR Kit
  • in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp™ seed PCR kit.

REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.

Features and Benefits

  • A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
  • Convenience- Hot-start feature in JumpStart™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
  • Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
  • High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 3

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

R4775-.15ML:4548174009437 + R4775-BULK: + R4775-1.2ML:4548174009444 + R4775PROC: + R4775-125ML-CO: + R4775-12ML-PW: + R4775-VAR: + R4775-PH: + R4775-12.5ML: + R4775-12ML-KC: + R3380-10ML-PW: + R4775-1.2ML-PW: + R3380-5ML-PW: + R4775-125ML:4548174009451 + R4268-5VL-PW: + R4775-.15ML-KC: + R4775-1.2ML-KC: + R4775-125ML-PW: + R4775-.15ML-PW: + R4775-12ML:4548174009468

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL2377
SLCH7798
SLCF1701
SLCF2922
SLCF4841

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Prescott T Leach et al.
eNeuro, 3(1) (2016-03-30)
Fragile X syndrome is a neurodevelopmental disorder characterized by mild-to-severe cognitive deficits. The complete absence of Fmr1 and its protein product in the mouse model of fragile X (Fmr1 KO) provides construct validity. A major conundrum in the field is
Phylogenetic placement, species delimitation, and cyanobiont identity of endangered aquatic Peltigera species (lichen-forming Ascomycota, Lecanoromycetes).
Miadlikowska J
American Journal of Botany, 101(7), 1141-1156 (2014)
Amelia R Hofstetter et al.
PloS one, 11(2), e0149864-e0149864 (2016-02-26)
The role of the reactive oxygen species-producing NADPH oxidase family of enzymes in the pathology of influenza A virus infection remains enigmatic. Previous reports implicated NADPH oxidase 2 in influenza A virus-induced inflammation. In contrast, NADPH oxidase 1 (Nox1) was
Gil Benedek et al.
PLoS neglected tropical diseases, 15(10), e0009821-e0009821 (2021-10-19)
Nodding syndrome (NS) is a catastrophic and enigmatic childhood epilepsy, accompanied by multiple neurological impairments and neuroinflammation. Of all the infectious, environmental and psychological factors associated with NS, the major culprit is Onchocerca Volvulus (Ov)-a parasitic worm transmitted to human
Rasmus H Jensen et al.
Journal of clinical microbiology, 49(2), 549-556 (2010-12-15)
Candida palmioleophila has previously been misidentified as C. famata or C. guilliermondii. We have investigated traditional and modern identification methods for the identification of this and related species. Forty-one clinical isolates previously identified as C. famata or C. guilliermondii and
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Articles

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

サンプル調製とゲル電気泳動のトラブルシューティング

SDS-PAGEサンプル調製の際に生じる課題の原因と対応策を特定し、電気泳動条件を最適化しましょう。

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

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Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

REDExtract-N-Amp™ Plant PCR Kit Protocol

REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.

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