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Merck

R5628

Hae III from Haemophilus aegyptius

Restriction Enzyme

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About This Item

CAS Number:
81295-18-3
UNSPSC Code:
12352204
MDL number:
MFCD00166568
EC Number:
3.1.21.4 (BRENDA, IUBMB)

Key Documents

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grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Application

HaeIII is a restriction enzyme that is used in molecular biology methods to cleave DNA at the recognition site 5′-GG/CC-3′ to generate DNA fragments with blunt termini.

Biochem/physiol Actions

Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.

Other Notes

Comment: Inefficient for single-stranded DNA cleavage.
Hae III requires optimal reaction conditions in order to avoid star activity.
Supplied with 10x Restriction Enzyme Buffer SM (B3158).

Physical form

Solution in 20 mM Tris-HCl, pH 7.7, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4°C

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Deleterious substance

pdsc

R5628-VAR: + R5628-BULK: + R5628-1KU:4548173295657 + R5628-2.5KU:4548173295664

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Certificates of Analysis (COA)

Lot/Batch Number
SLBQ0151V
SLBL3721V
SLBJ0699V
SLBJ0699
SLBD2732

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Stefano Fazi et al.
Environmental microbiology, 10(10), 2760-2772 (2008-07-23)
We studied the diversity, community composition and activity of the primary microbial colonizers of the water above freshly re-wetted sediments from a temporary river. Dried sediments, collected from Mulargia River (Sardinia, Italy), were covered with sterile freshwater in triplicate microcosms
Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius.
R W Blakesley et al.
The Journal of biological chemistry, 252(20), 7300-7306 (1977-10-25)
Beatrix Kotlan et al.
Journal of immunology (Baltimore, Md. : 1950), 175(4), 2278-2285 (2005-08-06)
The potential tumor-recognizing capacity of B cells infiltrating human breast carcinoma is an important aspect of breast cancer biology. As an experimental system, we used human medullary breast carcinoma because of its heavy B lymphocytic infiltration paralleled to a relatively
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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