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R5636

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
9014-25-9
UNSPSC Code:
12352106
NACRES:
NA.55
MDL number:
MFCD00132200
Form:
buffered aqueous solution
Quality level:
200

Key Documents

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grade

Molecular Biology

Quality Level

200

form

buffered aqueous solution

concentration

≥9 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
  • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
  • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
  • as a carrier to enhance recovery of RNA from small numbers of cells

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.

Preparation Note

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

R5636-1ML-PW: + R5636-5X1ML-PW: + R5636PROC: + R5636-PH: + R5636-BULK: + R5636-1ML: + R5636-5X1ML: + R5636-VAR:

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