R6148
RNase A solution
Synonym(s):
GenElute™ RNAse A
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About This Item
NACRES:
NA.52
UNSPSC Code:
12352204
Specific activity:
1500 units/mL, 3000 units/mL
Biological source:
bovine pancreas
Concentration:
20 mg/mL in Tris buffer
biological source
bovine pancreas
form
liquid
specific activity
1500 units/mL, 3000 units/mL
concentration
20 mg/mL in Tris buffer
technique(s)
DNA purification: suitable
storage temp.
room temp
Quality Level
Gene Information
bovine ... RNASE1(282340), RNASE1(280930)
Application
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Biochem/physiol Actions
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
General description
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Preparation Note
For usage requirements, please refer to the kit procedure.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
R6148PROC: + R6148-BULK: + R6148-.6ML-KC: + R6148-25ML: + R6148-VAR: + R6148-2ML: + R6148-.1ML-KC: + R6148-.2ML: + R6148-1.5ML: + R6148-2.5ML-KC: + R6148-2.5ML: + R6148-PH: + R5636-5X1ML-PW: + R6148-1.2ML: + R6148-.2ML-KC: + R6148-.6ML: + R6148-BULK-SP: + R6148-8ML: + R6148-.25ML-KC: + R6148-1.7ML: + R6148-25ML-CO: + R6148-8ML-KC: + R6148-2ML-KC: + R6148-4ML: + R6148-1.5ML-KC: + R6148-1.7ML-KC: + R6148-.25ML: + R6148-1.2ML-KC: + R6148-.4ML: + R6148-4ML-KC: + R6148-.1ML: + R6148-25ML-KC:
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