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Merck

R6626

Rhodamine B

≥95% purity (HPLC), powder

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
81-88-9
Molecular Weight:
479.01
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899386
EC Number:
201-383-9
MDL number:
MFCD00011931
Colour Index Number:
45170
Beilstein/REAXYS Number:
4091619

Key Documents

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Product Name

Rhodamine B, ≥95% (HPLC)

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

assay

≥95% (HPLC)

form

powder

technique(s)

titration: suitable

color

faint green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

0.79 g/cm3

ε (extinction coefficient)

104500-115800 at 542-554 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Rhodamine B has been used:
  • to represent fluorescence of tetra methyl rhodamine (TMR)
  • in the preparation of adhesive resin for confocal laser scanning microscopy
  • as a fluorescent dye in intrahemolymph dye dosing and retinal image acquisition
  • to depict brain-specific dye capture
  • for imaging targets

General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation. It is used as a biomarker in oral rabies vaccine.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN3952
SHBL5990
SHBL2114
MKCH5498
MKCF2710

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Fine depth resolution of two-photon absorption-induced photoacoustic microscopy using low-frequency bandpass filtering
YamaokaY, et al.
Optics Express, 19(14), 13365-13377 (2011)
Micromorphology of resin/dentin interfaces using 4th and 5th generation dual-curing adhesive/cement systems: a confocal laser scanning microscope analysis
Arrais CAG, et al.
The Journal of Adhesive Dentistry (2009)
5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells
HanGoo Kang
Nano-Micro Letters (2016)
Novel models for studying the blood-brain and blood-eye barriers in Drosophila
Nag S, et al.
The Blood-Brain and Other Neural Barriers, 357-369 (2011)
Palmitate mediated diacylglycerol accumulation causes endoplasmic reticulum stress, Plin2 degradation, and cell death in H9C2 cardiomyoblasts
AliAkoumi
Experimental Cell Research (2017)
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