R7509
RPMI-1640 Medium
With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered,suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
Product Name
RPMI-1640 Medium, Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
RPMI-1640 Medium has been used:
- to culture Mantle cell lymphoma cells
- to culture mouse podocytes
- in the extraction of whole bone marrow from rat Femur and tibia bones
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 Medium is suitable for culturing human normal and neoplastic leukocytes.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at clonal densities.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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