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Merck

S0266

Spermidine

BioReagent, Molecular Biology, suitable for cell culture, ≥98%

Synonym(s):

1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

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About This Item

Linear Formula:
NH2(CH2)3NH(CH2)4NH2
CAS Number:
124-20-9
Molecular Weight:
145.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24899434
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
MFCD00008229

Key Documents

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Product Name

Spermidine, BioReagent, Molecular Biology, suitable for cell culture, ≥98%

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

SMILES string

NCCCCNCCCN

grade

Molecular Biology

product line

BioReagent

assay

≥98%

form

solid or liquid

mol wt

145.25 (AMU)

technique(s)

cell culture | mammalian: suitable

refractive index

n20/D 1.479 (lit.)

density

0.925 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, and protease, none detected

storage temp.

room temp

Quality Level

200

Gene Information

rat ... Ppm1a(24666)

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Biochem/physiol Actions

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.

Disclaimer

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

General description

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Application

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.
Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

233.6 °F - closed cup

flash_point_c

112 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG8468
BCCG8129
BCCG9859
BCCG3088
BCCG1545

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Delivery of DNA to skin by particle bombardment.
Shixia Wang et al.
Methods in molecular biology (Clifton, N.J.), 245, 185-196 (2004-01-07)
Molecular Determinants of Metaplastic and Neoplastic Transformation in the Esophageal Epithelium
Progress in Molecular Biology and Translational Science, 96, 63-92 (2010)
Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs
Hogge GS, et al.
Cancer Gene Therapy, 6(1), 26-26 (1999)
Spermidine delays aging in humans
Madeo F, et al.
Aging (Albany. NY.), 10(8), 2209-2209 (2018)
Elumalai Sivamani et al.
Plant cell reports, 28(2), 213-221 (2008-11-19)
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded
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