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Merck

S1797

SOC Medium

For use in transformation

Synonym(s):

Super Optimal broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41106200

Key Documents

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Product Name

SOC Medium, For use in transformation

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

Quality Level

500

Related Categories

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF7416
SLCG7766
SLBW4174
SLCD1893
SLBZ9713

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Meg L Flanagan et al.
Methods in molecular biology (Clifton, N.J.), 378, 33-52 (2008-07-09)
As a source of recombinant antigen, soluble constant fragment (Fc) fusion proteins have become valuable reagents for immunotherapy and laboratory investigations. Additional applications for these reagents include flow cytometry, immunohistochemistry, and in vitro activity assays. To aid investigators in the
Kenneth Smith et al.
Nature protocols, 4(3), 372-384 (2009-02-28)
We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
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Articles

細菌形質転換プロトコル

コンピテントセルの増殖とその形質転換（DNAの取込み）の一般的なプロトコル。

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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