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Merck

S5150

Sodium chloride solution

5 M in H2O, BioReagent, Molecular Biology

Synonym(s):

Halite, NaCl, sodium chloride

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About This Item

Linear Formula:
NaCl
CAS Number:
7647-14-5
Molecular Weight:
58.44
UNSPSC Code:
12352302
NACRES:
NA.25
PubChem Substance ID:
24899641
EC Number:
231-598-3
Beilstein/REAXYS Number:
3534976
MDL number:
MFCD00003477
Grade:
Molecular Biology
Form:
liquid

Key Documents

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InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

SMILES string

[Na+].[Cl-]

grade

Molecular Biology

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H2O

technique(s)

ChIP: suitable, DNA extraction: suitable

Quality Level

200

impurities

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected, <1.0 EU/mL Endotoxin level, <16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm3

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm, heavy metals (as Pb): ≤5 ppm

suitability

suitable for Western blot, suitable for molecular biology

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

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Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA. It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.

Preparation Note

Prepared in 18 megohm water.

Other Notes

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S5150-CH: + S5150-BULK: + S5150-1.5ML:4548173210940 + S5150-8ML:4548173210971 + S5150-8ML-KC: + S5150-2ML-KC: + S5150-PH: + S5150-4L: + S5150-1.5ML-PW: + S5150-2ML:4548173210964 + S5150-H: + S5150-1.5ML-KC: + S5150PROC: + S5150-4L-PW: + S5150-2ML-PW: + S5150-VAR: + S5150-EW: + 80625: + S5150-1L-PW: + S5150-8ML-PW: + S5150-1L:4548173210957

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL5837
SLCL2574
SLCL6279
SLCJ8251
SLCL0212

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Vassileva G, et al.
The Journal of Endocrinology, 205(3), 225-232 (2010)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
Francis Kim et al.
JAMA, 311(1), 45-52 (2013-11-19)
Hospital cooling improves outcome after cardiac arrest, but prehospital cooling immediately after return of spontaneous circulation may result in better outcomes. To determine whether prehospital cooling improves outcomes after resuscitation from cardiac arrest in patients with ventricular fibrillation (VF) and
Laurent Dortet et al.
Journal of clinical microbiology, 52(7), 2359-2364 (2014-04-25)
Multidrug-resistant Acinetobacter baumannii isolates, particularly those that produce carbapenemases, are increasingly reported worldwide. The biochemically based Carba NP test, extensively validated for the detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified to detect carbapenemase production in
Alexander W Wyatt et al.
Genome biology, 15(8), 426-426 (2014-08-27)
Genomic analyses of hundreds of prostate tumors have defined a diverse landscape of mutations and genome rearrangements, but the transcriptomic effect of this complexity is less well understood, particularly at the individual tumor level. We selected a cohort of 25
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Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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