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Merck

S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
9031-54-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362

Key Documents

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Product Name

Sphingomyelinase from Bacillus cereus, buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

form

buffered aqueous glycerol solution

specific activity

≥100 units/mg protein (Lowry)

mol wt

34.2 kDa

foreign activity

Phospholipase C ≤1%

storage temp.

2-8°C

Quality Level

200

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

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Biochem/physiol Actions

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

General description

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
  • in lipoprotein modification for the generation of cholesterol crystals in vitro
  • to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
076M4045V
107M4103V
107M4102V
096M4114V
11160402

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Characterization of Sphingomyelin Phosphodiesterase Expression in Bumblebee (Bombus lantschouensis)
Han L, et al.
Journal of Insect Science, 18(5), 20-20 (2018)
Raymond Tan et al.
Experimental and molecular pathology, 94(1), 51-57 (2012-06-23)
The HIV-1 accessory protein Nef is considered to play an important role in the development of a podocyte phenotype in HIV-1 associated nephropathy. We hypothesized that Nef may be altering the podocyte phenotype both structurally and functionally. To elucidate the
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
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