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Merck

S9626

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-772-1
MDL number:
MFCD00132399
EC Number:
3.1.6.1 (BRENDA, IUBMB)

Key Documents

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type

Type H-1

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction
  • sulfatase assays
  • enzymatic hydrolysis
  • extract preparation for glucosinolate analysis

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

S9626-10KU: + S9626-VAR: + S9626-BULK: + S9626-5KU: + S9626-100KU:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL1880
SLCJ6301
SLCG4760
SLCH2450
SLCD2924

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A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)
Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction
Hirai T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 692(2), 375-388 (1997)
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Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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