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SRP9001

Leukemia Inhibitory Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, liquid, suitable for cell culture

Synonym(s):

Differentiation-stimulating factor, Emfilermin, LIF, Melanoma-derived LPL inhibitor

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
liquid
Mol wt:
20 kDa
Impurities:
≤1 EU/μg protein Endotoxin level
Product SRP9001 is not currently sold in your country.

Product Name

Leukemia Inhibitory Factor, human, recombinant, expressed in HEK 293 cells

biological source

human

Quality Segment

200

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

liquid

potency

≤0.5 ng/mL M1 cells proliferation inhibition EC50

mol wt

20 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg protein Endotoxin level

UniProt accession no.

P15018

storage temp.

−20°C

Gene Information

human ... LIF(3976)

General description

LIF is manufactured using an all-human production system, with no serum.
Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.
Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

Application

One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.
Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Physical form

This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.

Preparation Note

Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

SRP9001-10UG-PW: + SRP9001-10UG: + SRP9001-VAR: + SRP9001-BULK:

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